Experimental Cell Transplantation for Traumatic Spinal Cord Injury Regeneration: Intramedullar or Intrathecal Administration

  • Ana Alastrue-Agudo
  • Slaven Erceg
  • Marta Cases-Villar
  • Viviana Bisbal-Velasco
  • Richard J. Griffeth
  • Francisco Javier Rodriguez-Jiménez
  • Victoria Moreno-ManzanoEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1210)


Animal experimentation models are a necessary prerequisite to human trials for the use of regenerative medicine in the treatment of spinal cord injuries. Considerable effort is required for the generation of a consistent and reproducible methodology to incur an injury and evaluate the results. The traumatic contusion model has been accepted as a model that closely mimics a typical human traumatic injury, and here we detail step by step an approach to generate a reproducible lesion in rats. Acute cell transplantation by intramedullar or intrathecal administration is described for regenerative interventions. The same model is suitable to design subacute or chronic therapeutic approaches by interventions 1 week or 1 month after lesion.

Key words

Spinal cord injury Rat Cell transplantation Traumatic lesion Intramedullar Intrathecal 



This work was supported by FISS PI13/00319, Instituto de Salud Carlos III (Cofinanciación FEDER), and the Spanish Consolider Ion Channel Initiative [CSD 2008-00005] MICINN grants.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Ana Alastrue-Agudo
    • 1
  • Slaven Erceg
    • 2
  • Marta Cases-Villar
    • 1
  • Viviana Bisbal-Velasco
    • 1
  • Richard J. Griffeth
    • 1
  • Francisco Javier Rodriguez-Jiménez
    • 1
  • Victoria Moreno-Manzano
    • 1
    Email author
  1. 1.Neuronal and Tissue Regeneration LabCentro de Investigación Príncipe FelipeValenciaSpain
  2. 2.Retina Group, Cell Therapy and Regenerative MedicineCentro Andaluz de Biología Molecular y Medicina RegenerativaSevillaSpain

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