Experimental Cell Transplantation for Traumatic Spinal Cord Injury Regeneration: Intramedullar or Intrathecal Administration
Animal experimentation models are a necessary prerequisite to human trials for the use of regenerative medicine in the treatment of spinal cord injuries. Considerable effort is required for the generation of a consistent and reproducible methodology to incur an injury and evaluate the results. The traumatic contusion model has been accepted as a model that closely mimics a typical human traumatic injury, and here we detail step by step an approach to generate a reproducible lesion in rats. Acute cell transplantation by intramedullar or intrathecal administration is described for regenerative interventions. The same model is suitable to design subacute or chronic therapeutic approaches by interventions 1 week or 1 month after lesion.
Key wordsSpinal cord injury Rat Cell transplantation Traumatic lesion Intramedullar Intrathecal
This work was supported by FISS PI13/00319, Instituto de Salud Carlos III (Cofinanciación FEDER), and the Spanish Consolider Ion Channel Initiative [CSD 2008-00005] MICINN grants.
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