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Pancreas Development Ex Vivo: Culturing Embryonic Pancreas Explants on Permeable Culture Inserts, with Fibronectin-Coated Glass Microwells, or Embedded in Three-Dimensional Matrigel™

  • Hung Ping Shih
  • Maike SanderEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1210)

Abstract

Pancreas development is a complex and dynamic process orchestrated by cellular and molecular events, including morphogenesis and cell differentiation. As a result of recent explorations into possible cell-therapy-based treatments for diabetes, researchers have made significant progress in deciphering the developmental program of pancreas formation. In vitro pancreas organ culture systems provide a valuable tool for exploring the mechanisms of gene regulation, cellular behaviors, and cell differentiation. In this chapter, we review three common techniques for culturing embryonic pancreas explants. Each technique is suitable for different applications. Specifically, culturing embryonic pancreas on culture inserts provides an excellent platform to test the effects of chemical compounds. Conversely, when the embryonic pancreas is cultured in fibronectin-coated glass microwells, the system provides unique culture conditions to monitor organ growth and cellular dynamic events. Lastly, when the embryonic pancreas is embedded in Matrigel, organogenesis can be studied in a three-dimensional environment instead of limiting the analysis to one plane.

Key words

Pancreas Development Explants Differentiation Morphogenesis 

Notes

Acknowledgements

This work was supported by the NIH/NIDDK awards to M.S. (R01-DK078803 and R01-DK68471), and JDRF postdoctoral fellowships H.P.S. (3-2009-161).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Pediatrics and Cellular and Molecular Medicine, Pediatric Diabetes Research Center, UCSD Stem Cell ProgramUniversity of California San DiegoLa JollaUSA

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