The Generation and Maintenance of Rat Induced Pluripotent Stem Cells

  • Tomoyuki YamaguchiEmail author
  • Sanae Hamanaka
  • Hiromitsu Nakauchi
Part of the Methods in Molecular Biology book series (MIMB, volume 1210)


This chapter describes a newly developed method for generating and maintaining rat induced pluripotent stem cells (riPSCs). We first provide a detailed protocol for the generation of lentiviral vector carrying three reprogramming factors to produce high-quality riPSCs. This technique allows reprogramming of rat somatic cells to ground state with germ-line competence. Subsequently, we elaborate a detailed protocol for the generation of riPSCs from rat embryonic fibroblast (REF). Finally, the protocols for the optimal culture conditions of riPSCs and preparation of frozen stock are described. We also outline the advantages of generating riPSCs.

Key words

Rat induced pluripotent stem cells Lentiviral vector Dox inducible Germ-line competent GSK3 inhibitor MEK inhibitor 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Tomoyuki Yamaguchi
    • 1
    Email author
  • Sanae Hamanaka
    • 1
  • Hiromitsu Nakauchi
    • 1
  1. 1.Centre for Stem Cell Biology and Regenerative Medicine, Institute of Medical ScienceUniversity of TokyoMinato-kuJapan

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