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Proteomics of Endosomal Compartments from Plants Case Study: Isolation of Trans-Golgi Network Vesicles

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Plant Endosomes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1209))

Abstract

A detailed understanding of endomembrane processes and their biological roles is vital for a complete picture of plant growth and development; however their highly dynamic nature has complicated comprehensive and rigorous studies so far. Recent pioneering efforts have demonstrated that isolation of vesicles in their native state, paired with a quantitative identification of their cargo, offers a viable and practicable approach for the dissection of endomembrane trafficking pathways. The protocol presented in this chapter describes in detail the isolation of the SYP61 trans-Golgi network vesicles from Arabidopsis. With minor alterations, in a few key parameters, it can be adopted to yield a universal procedure for the broad spectrum of plant vesicles.

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Acknowledgements

This work was supported by the UCD Hellman Fellowship and the NSF-IOS 1258135 to GD.

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Authors and Affiliations

  1. Department of Plant sciences, University of California Davis, Asmundson Hall, One Shields Avenue, Davis, CA, 95616, USA

    Eunsook Park & Georgia Drakakaki

Authors
  1. Eunsook Park
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  2. Georgia Drakakaki
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Corresponding author

Correspondence to Georgia Drakakaki .

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Editors and Affiliations

  1. University of Wisconsin-Madison, Madison, Wisconsin, USA

    Marisa S. Otegui

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Park, E., Drakakaki, G. (2014). Proteomics of Endosomal Compartments from Plants Case Study: Isolation of Trans-Golgi Network Vesicles. In: Otegui, M. (eds) Plant Endosomes. Methods in Molecular Biology, vol 1209. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1420-3_14

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  • DOI: https://doi.org/10.1007/978-1-4939-1420-3_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1419-7

  • Online ISBN: 978-1-4939-1420-3

  • eBook Packages: Springer Protocols

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