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Examination of the Role of Galectins During In Vivo Angiogenesis Using the Chick Chorioallantoic Membrane Assay

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Galectins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1207))

Abstract

Angiogenesis is a complex multi-process involving various activities of endothelial cells. These activities are influenced in vivo by environmental conditions like interactions with other cell types and the microenvironment. Galectins play a role in several of these interactions and are therefore required for proper execution of in vivo angiogenesis. In this chapter we describe a method to study galectins and galectin inhibitors during physiologic and pathophysiologic angiogenesis in vivo using the chicken chorioallantoic membrane (CAM) assay.

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Author information

Authors and Affiliations

  1. Angiogenesis Laboratory Amsterdam, Department of Radiation Oncology, VU University medical center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands

    Esther A. Kleibeuker

  2. Angiogenesis Laboratory Amsterdam, Department of Medical Oncology, VU University medical center, De Boelelaan 1117, 1081 HV, Amsterdam, The Netherlands

    Iris A. E. Schulkens, Arjan W. Griffioen & Victor L. J. L. Thijssen

  3. Angiogenesis Laboratory Amsterdam, Department of Radiation Oncology, VU University medical center, Amsterdam, The Netherlands

    Kitty C. M. Castricum & Victor L. J. L. Thijssen

Authors
  1. Esther A. Kleibeuker
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  2. Iris A. E. Schulkens
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  3. Kitty C. M. Castricum
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  4. Arjan W. Griffioen
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  5. Victor L. J. L. Thijssen
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Corresponding author

Correspondence to Victor L. J. L. Thijssen .

Editor information

Editors and Affiliations

  1. Center for Transfusion and Cellular Therapies, Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, Georgia, USA

    Sean R. Stowell

  2. Department of Biochemistry, Emory University School of Medicine, Atlanta, GA, Georgia, USA

    Richard D. Cummings

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© 2015 Springer Science+Business Media New York

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Kleibeuker, E.A., Schulkens, I.A.E., Castricum, K.C.M., Griffioen, A.W., Thijssen, V.L.J.L. (2015). Examination of the Role of Galectins During In Vivo Angiogenesis Using the Chick Chorioallantoic Membrane Assay. In: Stowell, S., Cummings, R. (eds) Galectins. Methods in Molecular Biology, vol 1207. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1396-1_20

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  • DOI: https://doi.org/10.1007/978-1-4939-1396-1_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1395-4

  • Online ISBN: 978-1-4939-1396-1

  • eBook Packages: Springer Protocols

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