Abstract
It is now clear that long noncoding RNAs (lncRNAs) regulate a number of aspects of nuclear organization and gene expression. An important tool for the study of the distribution and function of lncRNAs is RNA fluorescence in situ hybridization (RNA-FISH). The protocols presented in this chapter describe this method in detail and also mention a number of critical points that must be considered when performing this technique.
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Acknowledgments
Research in the KVP lab is supported by grants from NIH/NIGMS (GM088252) and American Cancer Society (RSG-11-174-01-RMC). TH is an HHMI investigator.
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Tripathi, V., Fei, J., Ha, T., Prasanth, K.V. (2015). RNA Fluorescence In Situ Hybridization in Cultured Mammalian Cells. In: Carmichael, G. (eds) Regulatory Non-Coding RNAs. Methods in Molecular Biology, vol 1206. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1369-5_11
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DOI: https://doi.org/10.1007/978-1-4939-1369-5_11
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Publisher Name: Humana Press, New York, NY
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