Abstract
Nanospray desorption electrospray ionization (nano-DESI) is an ambient ionization technique that uses localized liquid extraction for mass spectrometry imaging of molecules on surfaces. Nano-DESI enables imaging of ionizable molecules from a sample in its native state without any special sample pretreatment. In this chapter we describe the protocol for nano-DESI imaging of thin tissue sections.
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References
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Laskin J, Heath BS, Roach PJ et al (2012) Tissue imaging using nanospray desorption electrospray ionization mass spectrometry. Anal Chem 84:141–148
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Acknowledgments
The protocol described in this chapter was developed as part of the Chemical Imaging Initiative at Pacific Northwest National Laboratory (PNNL). The research was conducted under the Laboratory Directed Research and Development Program at PNNL, a multiprogram national laboratory operated by Battelle for the US Department of Energy (DOE) under Contract DE-AC05-76RL01830. The experiments were performed at EMSL, a national scientific user facility sponsored by the DOE’s Office of Biological and Environmental Research and located at PNNL.
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Lanekoff, I., Laskin, J. (2015). Imaging of Lipids and Metabolites Using Nanospray Desorption Electrospray Ionization Mass Spectrometry. In: He, L. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 1203. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1357-2_10
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DOI: https://doi.org/10.1007/978-1-4939-1357-2_10
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-1357-2
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