Abstract
This chapter describes the processes of antibody (Ab) production, purification, conjugation to quantum dots (QDs), and the use of the conjugates produced in intracellular imaging of cell components and structures. Specifically, information is provided on the conjugation of carboxyl surface-terminated QDs to Abs via a one-step reaction using the water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC). The chapter details the process of conjugate optimization in terms of its final fluorescence and biological activity. The method described should guarantee the production of QD–Ab conjugates, which outperform classic organic fluorophore–Ab conjugates in terms of both image definition produced and the longevity of the imaging agent.
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East, D.A., Todd, M., Bruce, I.J. (2014). Quantum Dot–Antibody Conjugates via Carbodiimide-Mediated Coupling for Cellular Imaging. In: Fontes, A., Santos, B. (eds) Quantum Dots: Applications in Biology. Methods in Molecular Biology, vol 1199. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1280-3_5
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DOI: https://doi.org/10.1007/978-1-4939-1280-3_5
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