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Metabolite Fingerprinting by Capillary Electrophoresis-Mass Spectrometry

  • Antonia Garcia
  • Shama Naz
  • Coral Barbas
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1198)

Abstract

Changes in metabolite concentrations in response to specific diseases, treatments, diets, or other factors can be used to understand the complex mechanisms that control and regulate the human body and potentially detect the onset of disease prior to the observation of symptoms in a patient. Different analytical and chemometric platforms are necessary to detect as many metabolites as possible in different biological fluids.

Capillary electrophoresis (CE) coupled to mass spectrometry (MS) is a particularly attractive, although still not common, approach for metabolomics for the detection of mainly polar and ionic metabolites. Among its main features, CE provides the capability to separate complex mixtures with high resolution and minimum sample treatment. However, the routine, automated use of CE-MS is not without challenges. In this chapter we describe a well-tested method for fingerprinting serum and urine using CE-TOF-MS. We describe below a sensitive and quite robust method for metabolomics with CE-MS including sample treatment, separation conditions, instrumental setup, and identification of 76 metabolites in the profile. Useful advice for daily practice is also included for every step of the procedure.

Key words

Capillary electrophoresis Mass spectrometry Metabolomics Fingerprinting Serum Plasma Urine Biofluids 

Notes

Acknowledgements

Shama Naz receiving funding from the European Union Seventh Framework Programme [FP7/2007-2013] under grant agreement no. 264864. The authors gratefully also acknowledge the financial support from Ministerio de Economía y Competitividad (previously Ciencia y Tecnología) grant MCIT CTQ2011-23562.

References

  1. 1.
    Dunn WB, Ellis DI (2005) Metabolomics: Current analytical platforms and methodologies. Trends Anal Chem 24:285–294CrossRefGoogle Scholar
  2. 2.
    Barbas C, Moraes EP, Villasenor A (2010) Capillary electrophoresis as a metabolomics tool for non-targeted fingerprinting of biological samples. J Pharm Biomed Anal 53:122–129PubMedCrossRefGoogle Scholar
  3. 3.
    Monton MR, Soga T (2007) Metabolome analysis by capillary electrophoresis-mass spectrometry. J Chromatogr A 1168:237–246PubMedCrossRefGoogle Scholar
  4. 4.
    Ramautar R, Somsen GW, de Jong GJ (2009) CE-MS in metabolomics. Electrophoresis 30:276–291PubMedCrossRefGoogle Scholar
  5. 5.
    Ramautar R, Somsen GW, de Jong GJ (2013) CE-MS for metabolomics: developments and applications in the period 2010-2012. Electrophoresis 34:86–98PubMedCrossRefGoogle Scholar
  6. 6.
    Williams MD, Reeves R, Resar LS et al (2013) Metabolomics of colorectal cancer: past and current analytical platforms. Anal Bioanal Chem 405:5013–5030PubMedCrossRefGoogle Scholar
  7. 7.
    Williams BJ, Cameron CJ, Workman R et al (2007) Amino acid profiling in plant cell cultures: an inter-laboratory comparison of CE-MS and GC-MS. Electrophoresis 28: 1371–1379PubMedCrossRefGoogle Scholar
  8. 8.
    Ullsten S, Danielsson R, Bäckström D et al (2006) Urine profiling using capillary electrophoresis-mass spectrometry and multivariate data analysis. J Chromatogr A 1117: 87–93PubMedCrossRefGoogle Scholar
  9. 9.
    Staub A, Schappler J, Rudaz S et al (2009) CE–TOF/MS: fundamental concepts, instrumental considerations and applications. Electrophoresis 30:1610–1623PubMedCrossRefGoogle Scholar
  10. 10.
    Mayboroda OA, Neusub C, Pelzing M et al (2007) Amino acid profiling in urine by capillary zone electrophoresis—mass spectrometry. J Chromatogr A 1159:149–153PubMedCrossRefGoogle Scholar
  11. 11.
    Yu Z, Kastenmuller G, He Y et al (2011) Differences between human plasma and serum metabolite profiles. PLoS One 6:e21230PubMedCrossRefPubMedCentralGoogle Scholar
  12. 12.
    Atzei A, Atzori L, Moretti C et al (2011) Metabolomics in paediatric respiratory diseases and bronchiolitis. J Matern Fetal Neonatal Med 24:59–62PubMedCrossRefGoogle Scholar
  13. 13.
    Dunn WB, Broadhurst D, Begley P et al (2011) Human Serum Metabolome (HUSERMET) Consortium: Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry. Nat Protoc 6:1060–1083PubMedCrossRefGoogle Scholar
  14. 14.
    Naz S, Garcia A, Rusak M et al (2013) Method development and validation for rat serum fingerprinting with CE-MS: application to ventilator-induced-lung-injury study. Anal Bioanal Chem 405:4849–4858PubMedCrossRefGoogle Scholar
  15. 15.
    Moraes EP, Ruperez FJ, Plaza M et al (2011) Metabolomic assessment with CE-MS of the nutraceutical effect of Cystoseira spp extracts in an animal model. Electrophoresis 32: 2055–2062PubMedCrossRefGoogle Scholar
  16. 16.
    Dominguez-Alvarez J, Rodriguez-Gonzalo E, Hernandez-Mendez J et al (2011) Programmed nebulizing gas pressure for efficient and stable capillary electrophoresis mass spectrometry analysis of anionic compounds in positive separation mode. Anal Chem 83: 2834–2839PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.CEMBIO (Center for Metabolomics and Bioanalysis), Facultad De FarmaciaUniversidad CEU San PabloMadridSpain

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