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Sample Preparation Methods for LC-MS-Based Global Aqueous Metabolite Profiling

  • Antoni Beltran
  • Sara Samino
  • Oscar Yanes
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1198)

Abstract

Metabolite extraction is a key step in metabolomic analyses, particularly for untargeted studies. The extraction determines the types of metabolites that will be detected and the analytical platform to be used. In this chapter we describe two protocols aimed at detecting polar metabolites from biological samples; the first is aimed at detecting reduced species by LC/MS, and the second satisfies the requirements for both NMR and LC/MS analysis simultaneously.

Key words

LC-MS NMR Sample preparation Metabolomics Metabolite profiling 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Antoni Beltran
    • 1
    • 2
    • 3
  • Sara Samino
    • 1
    • 2
    • 3
  • Oscar Yanes
    • 1
    • 2
    • 3
  1. 1.Metabolomics PlatformSpanish Biomedical Research Center in Diabetes and Associated Metabolic Disorders (CIBERDEM)BarcelonaSpain
  2. 2.Centre for Omic Sciences (COS)Rovira i Virgili UniversityReusSpain
  3. 3.Institut d’Investigació Biomèdica Pere Virgili (IISPV)ReusSpain

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