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Mass Spectrometry in Metabolomics

Volume 1198 of the series Methods in Molecular Biology pp 29-41

Date:

LC-MS Profiling to Link Metabolic and Phenotypic Diversity in Plant Mapping Populations

  • Camilla B. HillAffiliated withAustralian Centre for Plant Functional Genomics (ACPFG), School of Botany, The University of Melbourne Email author 
  • , Antony BacicAffiliated withBio21 Molecular Science and Biotechnology Institute, The University of MelbourneARC Centre of Excellence in Plant Cell Walls and Metabolomics Australia, School of Botany, The University of Melbourne
  • , Ute RoessnerAffiliated withAustralian Centre for Plant Functional Genomics (ACPFG), School of Botany, The University of Melbourne

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Abstract

Numerous studies have revealed the extent of genetic, phenotypic, and metabolic variation between different plant cultivars/varieties. We present a specialized protocol for large-scale targeted and untargeted metabolite profiling for samples from large plant mapping populations using both reversed-phase and aqueous normal-phase LC-MS. This methodology provides a fast and combined targeted/nontargeted workflow as a powerful tool to discriminate related plant phenotypes and describes methods to combine mass features and agronomic traits to link phenotypic to metabolic traits independent of putative metabolite identities. This easily reproducible analytical strategy, in combination with a sophisticated data processing and analysis workflow, can be applicable to a wide range of plant mapping populations.

Key words

Liquid chromatography Mass spectrometry Quantitative trait locus mapping Mapping populations Metabolite profiling Metabolic trait Metabolomics Genomics