Metabolite Profiling by Direct Analysis in Real-Time Mass Spectrometry

  • Christina M. Jones
  • María Eugenia Monge
  • Facundo M. FernándezEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1198)


Untargeted metabolite profiling is a discovery tool for the identification of metabolites associated with the responses of perturbations to biological systems, such as a disease. Direct analysis in real-time mass spectrometry (DART MS) promises to be a powerful analytical technique for high-throughput metabolome analysis of human blood sera. Here, we describe the steps involved in untargeted blood sera metabolic profiling experiments using DART MS with two different sample introduction methods: probe-mode and transmission-mode geometries. Information regarding the optimization of different DART parameters that directly affect metabolite desorption and ionization, which thus influence sensitivity, is included.

Key words

Direct analysis in real time Metabolite profiling Serum metabolomics Transmission-mode DART Probe-mode DART Ambient mass spectrometry 



The authors acknowledge support from NSF grant CHE-0645094 and an OCRF Program Project Development grant. The authors also thank IonSense, Inc. for useful guidance on initial TM-DART experiments.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Christina M. Jones
    • 1
  • María Eugenia Monge
    • 1
  • Facundo M. Fernández
    • 1
    Email author
  1. 1.School of Chemistry and BiochemistryGeorgia Institute of TechnologyAtlantaUSA

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