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Multidimensional Mass Spectrometry-Based Shotgun Lipidomics

  • Miao Wang
  • Xianlin Han
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1198)

Abstract

Multidimensional mass spectrometry-based shotgun lipidomics (MDMS-SL) has become a foundational analytical technology platform among current lipidomics practices due to its high efficiency, sensitivity, and reproducibility, as well as its broad coverage. This platform has been broadly used to determine the altered content and/or composition of lipid classes, subclasses, and individual molecular species induced by diseases, genetic manipulations, drug treatments, and aging, among others. Herein, we briefly discuss the principles underlying this technology and present a protocol for routine analysis of many of the lipid classes and subclasses covered by MDMS-SL directly from lipid extracts of biological samples. In particular, lipid sample preparation from a variety of biological materials, which is one of the key components of MDMS-SL, is described in detail. The protocol for mass spectrometric analysis can readily be expanded for analysis of other lipid classes not mentioned as long as appropriate sample preparation is conducted, and should aid researchers in the field to better understand and manage the technology for analysis of cellular lipidomes.

Key words

Direct infusion Intrasource separation Lipidome Mass spectrometry Shotgun lipidomics 

Notes

Acknowledgements

This work was supported by National Institute on Aging Grants R01 AG31675 and intramural institutional research funds. Special thanks are expressed to Ms. Imee Tiu for her editorial assistance.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Diabetes and Obesity Research Center, Sanford-Burnham Medical Research InstituteOrlandoUSA

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