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Multiplexed, Quantitative, and Targeted Metabolite Profiling by LC-MS/MRM

  • Ru Wei
  • Guodong Li
  • Albert B. Seymour
Part of the Methods in Molecular Biology book series (MIMB, volume 1198)

Abstract

Targeted metabolomics, which focuses on a subset of known metabolites representative of biologically relevant metabolic pathways, is a valuable tool to discover biomarkers and link disease phenotypes to underlying mechanisms or therapeutic modes of action. A key advantage of targeted metabolomics, compared to discovery metabolomics, is its immediate readiness for extracting biological information derived from known metabolites and quantitative measurements. However, simultaneously analyzing hundreds of endogenous metabolites presents a challenge due to their diverse chemical structures and properties. Here we report a method which combines different chromatographic separation conditions, optimal ionization polarities, and the most sensitive triple-quadrupole MS-based data acquisition mode, multiple reaction monitoring (MRM), to quantitatively profile 205 endogenous metabolites in 10 min.

Key words

Targeted metabolomics Metabolomics MRM Metabolite quantitation Biomarker discovery Multiplexed chromatography 

Notes

Acknowledgements

The authors gratefully thank Jennifer L. Colangelo and Cynthia A. Drupa for kindly providing the rat plasma samples.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Proteomics, Translational ScienceBiogen IdecCambridgeUSA
  2. 2.Novartis Biomedical Research InstituteCambridgeUSA
  3. 3.Shire Human Genetic TherapiesLexingtonUSA

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