Mouse Genetics pp 289-312 | Cite as

Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development

  • Andrei M. Vacaru
  • Joseph Vitale
  • Johnathan Nieves
  • Margaret H. Baron
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1194)

Abstract

During the development of the hematopoietic system, at least eight distinct lineages are generated in the mouse embryo. Transgenic mice expressing fluorescent proteins at various points in the hematopoietic hierarchy, from hematopoietic stem cell to multipotent progenitors to each of the final differentiated cell types, have provided valuable tools for tagging, tracking, and isolating these cells. In this chapter, we discuss general considerations in designing a transgene and survey available fluorescent probes and methods for confirming and analyzing transgene expression in the hematopoietic systems of the embryo, fetus, and postnatal/adult animal.

Key words

Hematopoiesis Transgenic mice Knock-in Green fluorescent protein Fluorescent reporter 

Notes

Acknowledgements

We thank J. Barminko and G. Camprecios for their comments on the manuscript. Work in our laboratory was supported in part by grants to M.H.B. from the National Institutes of Health (RO1 HL62248 and, DK52191, and EB02209), the Roche Foundation for Anemia Research (grant 9699367999, cycle X), and the New York State Department of Health (NYSTEM grant N08G-024).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Andrei M. Vacaru
    • 1
    • 4
  • Joseph Vitale
    • 1
    • 4
  • Johnathan Nieves
    • 1
    • 4
  • Margaret H. Baron
    • 1
    • 2
    • 3
    • 4
    • 5
    • 6
  1. 1.Department of MedicineIcahn School of Medicine at Mount SinaiNew YorkUSA
  2. 2.Department of Developmental and Regenerative BiologyIcahn School of Medicine at Mount SinaiNew YorkUSA
  3. 3.Department of Oncological SciencesIcahn School of Medicine at Mount SinaiNew YorkUSA
  4. 4.The Tisch Cancer InstituteIcahn School of Medicine at Mount SinaiNew YorkUSA
  5. 5.The Black Family Stem Cell InstituteIcahn School of Medicine at Mount SinaiNew YorkUSA
  6. 6.Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount SinaiNew YorkUSA

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