Mass Cytometry Analysis of Human T Cell Phenotype and Function

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1193)

Abstract

Mass cytometry is a form of flow cytometry based on single-cell mass spectrometry that uses monoisotopic elemental labels to probe individual cells. Reduced cross talk between channels and an ability to measure >30 independent cellular parameters make this an attractive approach for high-dimensional analysis of cellular phenotypes and function. Here, methods of using this approach for the analysis of human T cell surface markers and intracellular cytokines are described.

Key words

Mass cytometry Cytometry by time-of-flight (CyTOF) Flow cytometry Intracellular cytokine staining 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Singapore Immunology Network (SIgN)Agency for Science Technology and Research (A-STAR)SingaporeSingapore

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