Mass Cytometry Analysis of Human T Cell Phenotype and Function
Mass cytometry is a form of flow cytometry based on single-cell mass spectrometry that uses monoisotopic elemental labels to probe individual cells. Reduced cross talk between channels and an ability to measure >30 independent cellular parameters make this an attractive approach for high-dimensional analysis of cellular phenotypes and function. Here, methods of using this approach for the analysis of human T cell surface markers and intracellular cytokines are described.
Key wordsMass cytometry Cytometry by time-of-flight (CyTOF) Flow cytometry Intracellular cytokine staining
We thank members of the Mark Davis, Garry Nolan, and Holden Maecker labs for their contributions of information about mass cytometry theory, usage, and experiences with various monoclonal antibodies leading to the development of this protocol. We also thank the mass cytometry platform users at the Singapore Immunology Network for assistance in validating this method, especially members of the Gennaro De Libero lab. Core funding through the Singapore Immunology Network supported this work.
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