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Induction of Passive EAE Using Myelin-Reactive CD4+ T Cells

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Book cover T-Helper Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1193))

Abstract

Experimental autoimmune encephalomyelitis (EAE) is an autoimmune disease of the central nervous system (CNS) often used as a model for the early inflammatory stages of multiple sclerosis and also as a model of organ-specific autoimmune disease.

This protocol describes the induction of passive EAE in mice, either using T cells isolated from mice primed with myelin antigens, or through the use of naïve TCR transgenic T cells activated in vitro in the presence of myelin-derived antigens.

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Authors and Affiliations

  1. MRC Centre for Inflammation Research and Centre for Multiple Sclerosis Research, Queen’s Medical Research Institute, University of Edinburgh, 47 Little France Crescent, Edinburgh, EH16 4TJ, UK

    Rhoanne C. McPherson, Helen E. Cambrook, Richard A. O’Connor & Stephen M. Anderton

Authors
  1. Rhoanne C. McPherson
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  2. Helen E. Cambrook
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  3. Richard A. O’Connor
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  4. Stephen M. Anderton
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Corresponding author

Correspondence to Stephen M. Anderton .

Editor information

Editors and Affiliations

  1. Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg University of Mainz, Mainz, Germany

    Ari Waisman

  2. University of Zurich Institute of Experimental Immunology, Zurich, Germany

    Burkhard Becher

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© 2014 Springer Science+Business Media New York

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McPherson, R.C., Cambrook, H.E., O’Connor, R.A., Anderton, S.M. (2014). Induction of Passive EAE Using Myelin-Reactive CD4+ T Cells. In: Waisman, A., Becher, B. (eds) T-Helper Cells. Methods in Molecular Biology, vol 1193. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1212-4_17

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  • DOI: https://doi.org/10.1007/978-1-4939-1212-4_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1211-7

  • Online ISBN: 978-1-4939-1212-4

  • eBook Packages: Springer Protocols

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