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Aldara-Induced Psoriasis-Like Skin Inflammation: Isolation and Characterization of Cutaneous Dendritic Cells and Innate Lymphocytes

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Book cover T-Helper Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1193))

Abstract

Psoriasis is a chronic auto-inflammatory skin disease of unknown etiology affecting millions of people worldwide. Dissecting the cellular networks and molecular signals promoting the development of psoriasis critically depends on appropriate animal models. Topical application of Aldara cream containing the Toll-like receptor (TLR)7-ligand Imiquimod induces skin inflammation and pathology in mice closely resembling plaque-type psoriasis in humans. The particular power of the Aldara model lies in examining the early events during psoriatic plaque formation, which is difficult to achieve in patients. Hence, recent reports using this model have challenged currently prevailing concepts concerning the pathophysiology of psoriasis. Here, we describe the induction and phenotype of Aldara-mediated dermatitis in mice and, in particular, analysis of the inflammatory cell infiltrate using flow cytometry.

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Author information

Authors and Affiliations

  1. Department of Immunology, Erasmus MC, University Medical Center Rotterdam, 3015 GE, Rotterdam, The Netherlands

    C. T. Wohn

  2. Institute of Experimental Immunology, University of Zürich, 8057, Zürich, Switzerland

    S. Pantelyushin

  3. Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg-University Mainz, Obere Zahlbacher Strasse 67, 55131, Mainz, Germany

    J. L. Ober-Blöbaum & B. E. Clausen

Authors
  1. C. T. Wohn
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  2. S. Pantelyushin
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  3. J. L. Ober-Blöbaum
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  4. B. E. Clausen
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Corresponding author

Correspondence to B. E. Clausen .

Editor information

Editors and Affiliations

  1. Institute for Molecular Medicine, University Medical Center of the Johannes Gutenberg University of Mainz, Mainz, Germany

    Ari Waisman

  2. University of Zurich Institute of Experimental Immunology, Zurich, Germany

    Burkhard Becher

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© 2014 Springer Science+Business Media New York

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Wohn, C.T., Pantelyushin, S., Ober-Blöbaum, J.L., Clausen, B.E. (2014). Aldara-Induced Psoriasis-Like Skin Inflammation: Isolation and Characterization of Cutaneous Dendritic Cells and Innate Lymphocytes. In: Waisman, A., Becher, B. (eds) T-Helper Cells. Methods in Molecular Biology, vol 1193. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1212-4_16

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  • DOI: https://doi.org/10.1007/978-1-4939-1212-4_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1211-7

  • Online ISBN: 978-1-4939-1212-4

  • eBook Packages: Springer Protocols

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