Microscopy Assays for Evaluation of Mast Cell Migration and Chemotaxis
A better understanding of the molecular mechanisms leading to mast cell migration and chemotaxis is the long-term goal in mast cell research and is essential for comprehension of mast cell function in health and disease. Various techniques have been developed in recent decades for in vitro and in vivo assessment of mast cell motility and chemotaxis. In this chapter three microscopy assays facilitating real-time quantification of mast cell chemotaxis and migration are described, focusing on individual cell tracking and data analysis.
Key wordsMast cells Cell migration Chemotaxis Chemokine Chemoattractant
This work was supported in part by Grants P302-14-09807S, P305-14-00703S, 301/09/1826, P302/10/1759, P302/12/G101, and P302/12/1673 from the Czech Science Foundation, project LD13015 and LD12073 from the Ministry of Education, Youth and Sport of the Czech Republic. We also acknowledge support of the COST Action BM1007 Mast Cells and Basophils—Targets for Innovative Therapies as well as Institutional support RVO 68378050. M.B. and Z.H. were supported in part by the Faculty of Science, Charles University, Prague.
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