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Tissue Morphogenesis pp 115–122Cite as

A Multiplex Fluorescent In Situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis

Part of the Methods in Molecular Biology book series (MIMB,volume 1189)

Abstract

Fluorescent in situ hybridization (FISH) is a common inmunohistochemical method used to examine the distribution of RNAs in tissue samples. In mosaic tissues composed of a mixed population of wild-type and loss-of- or gain-of-function mutant cells, FISH allows comparison of the effect of the perturbation on gene expression patterns in a mutant cell and its wild-type neighbors. Here, we provide a protocol for the detection of RNA in Drosophila mosaic follicular epithelia, where the mosaic analysis with a repressible cell marker (MARCM) technique is used for expression of transgenes.

Key words

  • Drosophila melanogaster
  • Oogenesis
  • Follicle cells
  • Mosaic analysis with a repressible cell marker (MARCM)
  • Fluorescent in situ hybridization (FISH)

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  • DOI: 10.1007/978-1-4939-1164-6_8
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Author information

Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ, USA

    Lily S. Cheung

  2. Carl Icahn Laboratory, Lewis-Sigler Institute for Integrative Genomics, Princeton University, Washington Road, Princeton, NJ, USA

    Stanislav Shvartsman Ph.D.

Authors
  1. Lily S. Cheung
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  2. Stanislav Shvartsman Ph.D.
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Corresponding author

Correspondence to Stanislav Shvartsman Ph.D. .

Editor information

Editors and Affiliations

  1. Chemical & Biological Engineering, Princeton University, New Jersey, USA

    Celeste M. Nelson

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Cheung, L.S., Shvartsman, S. (2015). A Multiplex Fluorescent In Situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis. In: Nelson, C. (eds) Tissue Morphogenesis. Methods in Molecular Biology, vol 1189. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1164-6_8

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  • DOI: https://doi.org/10.1007/978-1-4939-1164-6_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1163-9

  • Online ISBN: 978-1-4939-1164-6

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