Abstract
Fluorescent in situ hybridization (FISH) is a common inmunohistochemical method used to examine the distribution of RNAs in tissue samples. In mosaic tissues composed of a mixed population of wild-type and loss-of- or gain-of-function mutant cells, FISH allows comparison of the effect of the perturbation on gene expression patterns in a mutant cell and its wild-type neighbors. Here, we provide a protocol for the detection of RNA in Drosophila mosaic follicular epithelia, where the mosaic analysis with a repressible cell marker (MARCM) technique is used for expression of transgenes.
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Cheung, L.S., Shvartsman, S. (2015). A Multiplex Fluorescent In Situ Hybridization Protocol for Clonal Analysis of Drosophila Oogenesis. In: Nelson, C. (eds) Tissue Morphogenesis. Methods in Molecular Biology, vol 1189. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1164-6_8
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DOI: https://doi.org/10.1007/978-1-4939-1164-6_8
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-1163-9
Online ISBN: 978-1-4939-1164-6
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