Intracellular Signaling of CTLs
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Extracellular signals are transmitted to intracellular targets thanks to a complex network of interacting proteins that regulate a large number of cellular processes. The signaling mechanisms that occur in CTLs are initiated by T cell receptors (TCRs) that recognize antigens presented in the groove of class I histocompatibility (MHC) molecules of target cells. The strength of this interaction and the consequent activation of intracellular mechanisms determine the fate of the developing T cells. The intracellular mediators of activation can be easily detected using a classical methodology such as western blot. Here we describe this technique bearing particular attention to these mediators using SNAP i.d., a newly developed protein detection system.
Key wordsCTL intracellular signaling Protein phosphorylation SDS-polyacrylamide gel electrophoresis SNAP i.d. immunoblotting
This work was supported by grants from the MiUR (PON-REC ONEV 134/2011; FIRB RBAP11B2SX) and Regione Puglia (BISIMANE project, H31D08000030007).