In Vitro\Ex Vivo Generation of Cytotoxic T Lymphocytes

  • Margherita Gigante
  • Elena RanieriEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1186)


The in vitro generation of human cytotoxic T lymphocytes (CTL) is a reliably approach useful not only to assess intrinsic CD8+ T cell responses in individuals but also to screen immunogenic antigens that could be considered as candidates for adoptive immunotherapeutic approaches. In vitro methods to expand CTL require culturing naïve T cells with antigen-presenting cells (APC) as stimulator cells that express specific antigens. Here, we describe the protocol for generating CTL against target antigens presented by monocyte-derived dendritic cells (DCs).

Key words

CD8+ T cell Cytotoxicity Dendritic cells Peptides Cancer 



This work was supported by Progetto Strategico Regione Puglia grant (E.R., 2008), Ministero dell’Istruzione, dell’Università e della Ricerca (MiUR) FIRB, CAROMICS grant (E.R., 2011), and a Postdoctoral Fellowship by MiUR, PROGRAMMA OPERATIVO NAZIONALE (PON) RICERCA E COMPETITIVITÀ 2007–2013 PONa3_00395 “BIOSCIENCES AND HEALTH” (B&H) (M.G., 2013). The authors declare no conflicts of interest.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Nephrology, Dialysis and Transplantation Unit, Department of Emergency and Organ Transplantation, PoliclinicoUniversity of Bari “A. Moro”BariItaly
  2. 2.Department of Surgical and Medical, Sciences, School of Medicine, Ospedali RiunitiUniversity of FoggiaFoggiaItaly

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