In Vitro\Ex Vivo Generation of Cytotoxic T Lymphocytes
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The in vitro generation of human cytotoxic T lymphocytes (CTL) is a reliably approach useful not only to assess intrinsic CD8+ T cell responses in individuals but also to screen immunogenic antigens that could be considered as candidates for adoptive immunotherapeutic approaches. In vitro methods to expand CTL require culturing naïve T cells with antigen-presenting cells (APC) as stimulator cells that express specific antigens. Here, we describe the protocol for generating CTL against target antigens presented by monocyte-derived dendritic cells (DCs).
Key wordsCD8+ T cell Cytotoxicity Dendritic cells Peptides Cancer
This work was supported by Progetto Strategico Regione Puglia grant (E.R., 2008), Ministero dell’Istruzione, dell’Università e della Ricerca (MiUR) FIRB, CAROMICS grant (E.R., 2011), and a Postdoctoral Fellowship by MiUR, PROGRAMMA OPERATIVO NAZIONALE (PON) RICERCA E COMPETITIVITÀ 2007–2013 PONa3_00395 “BIOSCIENCES AND HEALTH” (B&H) (M.G., 2013). The authors declare no conflicts of interest.
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