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Experimental Model for Studying the Involvement of Regulatory Cytotoxic T Cells in Bone Resorption

  • Giacomina BrunettiEmail author
  • Angela Oranger
  • Silvia Colucci
  • Maria Grano
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1186)

Abstract

T cells may affect the activity of osteoclasts, the bone resorbing cells. In particular, recently authors focalize their attention on CD8+ T cells, demonstrating that murine pre-osteoclasts can recruit naïve CD8+ T cells and induce them to differentiate in regulatory cytotoxic T cells (TcREG), which in turn may suppress osteoclast formation and activity. Thus, here we describe the methodological approach needed to obtain TcREG and to evaluate TcREG effects on murine osteoclast activity and formation in an in vitro experimental model.

Key words

T cells Osteoclastogenesis FoxP3+CD8+ T cells TRAP staining Resorption assay 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Giacomina Brunetti
    • 1
    • 2
    Email author
  • Angela Oranger
    • 1
    • 2
  • Silvia Colucci
    • 1
    • 2
  • Maria Grano
    • 1
    • 2
  1. 1.Section of Human Anatomy and Histology, Department of Basic Medical Sciences, Neurosciences, and Sense OrgansUniversity of BariBariItaly
  2. 2.PoliclinicoBariItaly

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