Two-Dimensional Gel Electrophoresis Approach for CTL Phosphoproteome Analysis

  • Maria Teresa RocchettiEmail author
  • Massimo Papale
  • Loreto Gesualdo
Part of the Methods in Molecular Biology book series (MIMB, volume 1186)


Phosphorylation of proteins plays a pivotal role in signal transduction processes, and it is a key regulator of many biological cell functions. Various strategies have been proposed for the study of phosphoproteome; most of them require a multi-step analysis and sophisticated equipment. Here we describe the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) analysis of PBMC phosphoproteome using as preliminary enrichment step a simple phosphoprotein isolation by lanthanum chloride. This strategy can be most certainly applied to study the phosphoproteome of CTLs isolated from PBMCs. The phosphoproteome analysis of PBMCs, as well as of CTLs, may help to reveal the signaling pathways essential to their biological role in health and disease.

Key words

Phosphoproteome Two-dimensional gel electrophoresis Lanthanum chloride PBMC CTL 



The study was supported by the Grant of the Italian Ministry of Research and Education (FIRB2010-CAROMICS) to L.G. and E.R.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Maria Teresa Rocchetti
    • 2
    Email author
  • Massimo Papale
    • 1
  • Loreto Gesualdo
    • 2
  1. 1.Core Facility of Proteomics and Mass Spectrometry, Department of Surgery and Medical SciencesUniversity of FoggiaFoggiaItaly
  2. 2.Department of Emergency and Organ TransplantationUniversity of BariBariItaly

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