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Proteomic Approaches by SELDI and MALDI-TOF/MS for CTL Analysis

  • Massimo PapaleEmail author
  • Maria Teresa Rocchetti
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1186)

Abstract

Extracellular stimuli activate, on target cells, a number of signal transduction processes regulating gene expression and the function and/or synthesis of the proteins. In order to highlight the slight changes of the quantity and quality of the proteome it is essential to optimize preparative strategies able to improve the signals of the less expressed proteins and to standardize the use of high-throughput techniques useful to detect them. We describe a complete workflow useful to enrich, from PBMC protein extracts and extrapolated to their subpopulations, the low-molecular-weight proteins and peptides and to detect them by SELDI-TOF protein profiling. The described protocol can also be applied to MALDI-TOF/MS instruments in order to obtain fast, reproducible, and high-quality protein profiles.

Key words

SELDI-TOF/MS MALDI-TOF/MS CTL PBMC Proteomics Biomarkers 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Surgery and Medical SciencesUniversity of FoggiaFoggiaItaly
  2. 2.Department of Emergency and Organ TransplantationUniversity of Bari Aldo MoroBariItaly

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