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Monitoring Notch Activity in the Mouse

  • Swananda Marathe
  • Lavinia AlberiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1187)

Abstract

Several laboratories have developed genetic methods to monitor Notch activity in developing and adult mice. These approaches have been useful in identifying Notch signaling with high temporal and spatial resolution. This research has contributed substantially to our understanding of the role of Notch in cell specification and cellular physiology. Here, we present two protocols to monitor Notch activity in the mouse brain: (1) by intraventricular electroporation and (2) by intracranial viral injections of Notch reporter constructs. These methods allow monitoring of Notch signaling in specific brain regions from development to adulthood. In addition, using the appropriate modifications, the Notch reporter systems can also be used to monitor Notch activity in other organs of the mouse such as retina, skin, skeletal muscle, and cancer cells.

Key words

Notch activity reporter CBF1 Hes genes In utero electroporation Intracranial viral injection 

Notes

Acknowledgments

We would like to thank Nicholas Gaiano and Kenichi Mizutani for allowing us to reprint one of their results and for helping with the intraventricular electroporation technique. We would also like to thank Mauro Giacca and Lorena Zentilin for packaging the AAVs. This work is supported by the Swiss National Foundation, the Synapsis Foundation for Alzheimer’s Research, and Swiss Heart Association.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Unit of Anatomy, Department of MedicineUniversity of FribourgFribourgSwitzerland

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