Application of Anti-Ligand Antibodies to Inhibit Notch Signaling

  • Jun-ichiro Koga
  • Masanori AikawaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1187)


Emerging evidence suggests that Notch signaling not only regulates biological processes during development but also participates in the pathogenesis of various diseases in adults, including tumor angiogenesis, hematopoietic malignancies, and cardiometabolic syndromes. Notch signaling involves several ligands and receptors that have unique and overlapping functions. Therefore, blocking function of a ligand or receptor with a neutralizing antibody is a useful approach to examine the specific role of each Notch component. In addition, administration of Notch signaling blocking antibodies in experimental animals offers important insights into clinical translation of Notch biology. In this chapter, we describe examples of in vitro and in vivo loss-of-function experiments with blockade of Notch ligands, particularly Delta-like ligand 4 (Dll4).

Key words

Notch ligand Blocking antibody Delta-like ligand 4 Luciferase assay Nucleofection 



This work was supported in part by a National Institutes of Health grant R01HL107550 to M.A.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.The Center for Excellence in Vascular BiologyBrigham and Women’s Hospital, Harvard Medical SchoolBostonUSA
  2. 2.The Center for Excellence in Vascular Biology and Center for Interdisciplinary Cardiovascular SciencesBrigham and Women’s Hospital, Harvard Medical SchoolBostonUSA

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