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Proteomic Analysis of the Notch Interactome

  • K. G. Guruharsha
  • Kazuya Hori
  • Robert A. Obar
  • Spyros Artavanis-TsakonasEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1187)

Abstract

Recent large-scale studies have provided a global description of the interactome—the whole network of protein interactions in a cell or an organism—for several model organisms. Defining protein interactions on a proteome-wide scale has led to a better understanding of the cellular functions of many proteins, especially those that have not been studied by classical molecular genetic approaches. Here we describe the resources, methods, and techniques necessary for generation of such a proteome-scale interactome in a high throughput manner. These procedures will also be applicable to low or medium throughput focused studies aimed at understanding interactions between members of specific pathways such as Notch signaling.

Key words

Notch signaling Proteomics Protein interactions Genetic interactions Interactome Integrated network 

Notes

Acknowledgements

This work was supported by NIH grants 5R01HG003616, NS26084, and CA98402 (S.A-T.), as well as a JSPS Postdoctoral Fellowship for Research Abroad (K.H.), and generation of the clone set was supported by a grant from the National Human Genome Research Institute (NHGRI P41HG3487) to our collaborator Susan Celniker.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • K. G. Guruharsha
    • 1
    • 2
  • Kazuya Hori
    • 1
  • Robert A. Obar
    • 1
  • Spyros Artavanis-Tsakonas
    • 1
    Email author
  1. 1.Department of Cell BiologyHarvard Medical SchoolBostonUSA
  2. 2.Biogen IdecCambridgeUSA

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