Notch Signaling Assays in Drosophila Cultured Cell Lines
Signaling assays in Drosophila cell lines are a valuable method for investigating whether other proteins influence the function of the Notch pathway and for assessing whether specific enhancers or genes are regulated by Notch. In this chapter, we will describe two different types of assays that can be used to monitor Notch activation in Kc167 and S2 cells. One involves activating Notch in cultured cells and measuring the change in endogenous gene expression levels. The other uses luciferase reporters and measures their response to Notch, by co-transfecting with NICD.
Key wordsNotch Drosophila cell line EDTA/EGTA treatment Real-time PCR E(spl) genes NRE Enhancer Dual-luciferase assay Luciferase reporter Cell transfection