Hematopoietic Stem Cell Protocols pp 155-164

Part of the Methods in Molecular Biology book series (MIMB, volume 1185) | Cite as

Metabolic Characterization of Hematopoietic Stem Cells

  • Fatih Kocabas
  • Junke Zheng
  • Chengcheng Zhang
  • Hesham A. Sadek
Protocol

Abstract

An important feature of stem cells is their maintenance in their respective hypoxic niche. Survival in this low-oxygen microenvironment requires significant metabolic adaptation. We demonstrated that mouse HSCs utilize glycolysis instead of mitochondrial oxidative phosphorylation to meet their energy demands. We have adapted various tools for characterization of the metabolic properties of hematopoietic stem cells (HSCs). These techniques include flow cytometric profiling of HSCs based on mitochondrial potential and NADH fluorescence as well as measurement of ATP content, oxygen consumption rate, and glycolytic flux in purified HSCs.

Key words

Hematopoietic stem cells HSC Stem cell metabolism Reactive oxygen species ROS Glycolysis ATP content Oxygen consumption NADH Glycolytic flux Lactate 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Fatih Kocabas
    • 1
  • Junke Zheng
    • 2
  • Chengcheng Zhang
    • 3
  • Hesham A. Sadek
    • 4
  1. 1.Department of Education, and Texas Institute of Biotechnology, Education and ResearchNorth American UniversityHoustonUSA
  2. 2.Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of EducationShanghai Jiao-Tong University School of MedicineShanghaiChina
  3. 3.Departments of Physiology and Developmental BiologyUniversity of Texas Southwestern Medical CenterDallasUSA
  4. 4.Department of Internal Medicine, Division of CardiologyUniversity of Texas Southwestern Medical CenterDallasUSA

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