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Metabolic Characterization of Hematopoietic Stem Cells

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Hematopoietic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1185))

Abstract

An important feature of stem cells is their maintenance in their respective hypoxic niche. Survival in this low-oxygen microenvironment requires significant metabolic adaptation. We demonstrated that mouse HSCs utilize glycolysis instead of mitochondrial oxidative phosphorylation to meet their energy demands. We have adapted various tools for characterization of the metabolic properties of hematopoietic stem cells (HSCs). These techniques include flow cytometric profiling of HSCs based on mitochondrial potential and NADH fluorescence as well as measurement of ATP content, oxygen consumption rate, and glycolytic flux in purified HSCs.

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References

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Acknowledgements

This work was supported by grants from the American Heart Association (Grant-in-Aid), the Gilead Research Scholars Program in Cardiovascular Disease, Foundation for Heart Failure Research, NY, and the NIH (1R01HL115275-01).

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Authors and Affiliations

  1. Department of Education, and Texas Institute of Biotechnology, Education and Research, North American University, 3203 N. Sam Houston Pkwy W, Houston, TX, 77038, USA

    Fatih Kocabas

  2. Key Laboratory of Cell Differentiation and Apoptosis of Chinese Ministry of Education, Shanghai Jiao-Tong University School of Medicine, Chongqing South Road 280, Shanghai, 200025, China

    Junke Zheng

  3. Departments of Physiology and Developmental Biology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX, 75390-8573, USA

    Chengcheng Zhang

  4. Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, 5323 Harry Hines Boulevard, Dallas, TX, 75390-8573, USA

    Hesham A. Sadek

Authors
  1. Fatih Kocabas
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  2. Junke Zheng
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  3. Chengcheng Zhang
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  4. Hesham A. Sadek
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Corresponding author

Correspondence to Hesham A. Sadek .

Editor information

Editors and Affiliations

  1. Department of Pediatrics, Aflac Cancer and Blood Disorders Center Emory University School of Medicine, Atlanta, Georgia, USA

    Kevin D. Bunting

  2. Department of Pediatrics, Emory University School of Medicine Aflac Cancer & Blood Disorders Ctr, Atlanta, Georgia, USA

    Cheng-Kui Qu

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© 2014 Springer Science+Business Media New York

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Kocabas, F., Zheng, J., Zhang, C., Sadek, H.A. (2014). Metabolic Characterization of Hematopoietic Stem Cells. In: Bunting, K., Qu, CK. (eds) Hematopoietic Stem Cell Protocols. Methods in Molecular Biology, vol 1185. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1133-2_10

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  • DOI: https://doi.org/10.1007/978-1-4939-1133-2_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-1132-5

  • Online ISBN: 978-1-4939-1133-2

  • eBook Packages: Springer Protocols

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