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Whole-Cell Patch-Clamp Recordings in Freely Moving Animals

  • Albert K. LeeEmail author
  • Jérôme EpszteinEmail author
  • Michael BrechtEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1183)

Abstract

The patch-clamp technique and the whole-cell measurements derived from it have greatly advanced our understanding of the coding properties of individual neurons by allowing for a detailed analysis of their excitatory/inhibitory synaptic inputs, intrinsic electrical properties, and morphology. Because such measurements require a high level of mechanical stability they have for a long time been limited to in vitro and anesthetized preparations. Recently, however, a considerable amount of effort has been devoted to extending these techniques to awake restrained/head-fixed preparations allowing for the study of the input–output functions of neurons during behavior. In this chapter we describe a technique extending patch-clamp recordings to awake animals free to explore their environments.

Key words

Intracellular recording Awake-behaving Hippocampus CA1 

Notes

Acknowledgments

This work was supported by an EMBO Long Term Fellowship and HHMI (to A. K. L.); a Human Frontier Science Program Long Term Fellowship, INSERM, Agence Nationale de la Recherche (grant ANR-09-BLAN-0259-01 and ANR-10-R11014AA), Région PACA (project EPIVIRT), A*MIDEX project (ANR-11-IDEX-0001-02) funded by the «Investissements d’Avenir» French Government program (to J.E.); Humboldt Universität zu Berlin, the Bernstein Center for Computational Neuroscience Berlin, the German Federal Ministry of Education and Research (BMBF, Förderkennzeichen 01GQ1001A), NeuroCure, a European Research Council grant, and the Gottfried Wilhelm Leibniz Prize of the DFG (to M.B).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Janelia Farm Research CampusHoward Hughes Medical InstituteAshburnUSA
  2. 2.Institut National de la Santé et de la Recherche Médicale (INSERM U901)MarseilleFrance
  3. 3.Aix-Marseille UniversityMarseilleFrance
  4. 4.Institut de Neurobiologie de la Méditerranée (INMED)Marseille Cedex 09France
  5. 5.Bernstein Center for Computational Neuroscience BerlinHumboldt University of BerlinBerlinGermany
  6. 6.Cluster of Excellence NeuroCureCharité-Universitätsmedizin BerlinBerlinGermany

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