Whole-Cell Patch-Clamp Recordings in Freely Moving Animals
The patch-clamp technique and the whole-cell measurements derived from it have greatly advanced our understanding of the coding properties of individual neurons by allowing for a detailed analysis of their excitatory/inhibitory synaptic inputs, intrinsic electrical properties, and morphology. Because such measurements require a high level of mechanical stability they have for a long time been limited to in vitro and anesthetized preparations. Recently, however, a considerable amount of effort has been devoted to extending these techniques to awake restrained/head-fixed preparations allowing for the study of the input–output functions of neurons during behavior. In this chapter we describe a technique extending patch-clamp recordings to awake animals free to explore their environments.
Key wordsIntracellular recording Awake-behaving Hippocampus CA1
This work was supported by an EMBO Long Term Fellowship and HHMI (to A. K. L.); a Human Frontier Science Program Long Term Fellowship, INSERM, Agence Nationale de la Recherche (grant ANR-09-BLAN-0259-01 and ANR-10-R11014AA), Région PACA (project EPIVIRT), A*MIDEX project (ANR-11-IDEX-0001-02) funded by the «Investissements d’Avenir» French Government program (to J.E.); Humboldt Universität zu Berlin, the Bernstein Center for Computational Neuroscience Berlin, the German Federal Ministry of Education and Research (BMBF, Förderkennzeichen 01GQ1001A), NeuroCure, a European Research Council grant, and the Gottfried Wilhelm Leibniz Prize of the DFG (to M.B).
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