Multiphoton Imaging Approaches for Studying Striatal Dendritic Excitability
As the main input nucleus to the basal ganglia, the striatum is responsible for receiving and integrating highly convergent afferents to ultimately guide action selection and movement initiation. Although the majority of this synaptic integration occurs in the dendrites of striatal projection neurons (SPNs), their thin diameter makes them inaccessible with traditional recording electrodes. Recent advances in optical imaging technologies have allowed us and others to start lifting the veil on the mechanisms governing synaptic integration in the striatum by enabling direct dendritic measurements and manipulations. Here we describe how our lab has approached combining 2-photon imaging and photolysis with electrophysiological recordings to study dendritic excitability and synaptic integration in the striatum.
Key words2-Photon imaging 2-Photon uncaging Calcium imaging Striatum Dendritic morphology
We thank Drs. David Wokosin and Michelle Day for their invaluable contributions to establishing this technology in our laboratory. This work was funded by CHDI.
- 4.Wilson CJ (1992) Dendritic morphology, inward rectification and the functional properties of neostriatal neurons. In: McKenna TM, Davis JL, Zornetzer SF (eds) Single neuron computation (neural networks: foundations to applications), 1st edn. Academic Press Professional Inc, San Diego, CA, pp 141–171CrossRefGoogle Scholar
- 9.Plotkin JL, Guzman JN, Schwarz N et al (2011) Optical approaches to studying the basal ganglia. In: Lane EL, Dunnett SB (eds) Animal models of movement disorders: Volume I, vol 61, Neuromethods. Springer Science + Business Media, New York, pp 191–220. doi: 10.1007/978-1-61779-298-4_10 CrossRefGoogle Scholar