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Chromosomal Aberration Test Utilities In Vitro and In Vivo

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Genotoxicity and DNA Repair

Part of the book series: Methods in Pharmacology and Toxicology ((MIPT))

Abstract

Human populations are frequently exposed to several mutagenic agents that have the potential to damage the DNA, and this, in many cases, may result in the formation of chromosomal aberrations (CAs). CAs are recognized as an important biomarker of human exposure, being a very important tool for environmental biomonitoring. Although there are several types, little is known about the mechanisms involved in the processing of induced lesions in DNA and how these could result in CAs. Thus, cytogenetics and molecular cytogenetics are tools of great importance for identifying these agents, the conditions that can exercise their mutagenic potential, and their action mechanism. This chapter discusses the history of CA formation and some cytogenetic protocols that may be used to perform the chromosomal aberration test in in vivo and in vitro studies.

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Authors and Affiliations

  1. Centro de Ciências Biológicas e da Saúde, Universidade do Estado do Pará, Pará, Brazil

    Ana Paula A. Guimarães & Patrícia L. Lima

  2. Laboratório de Citogenética Humana, Instituto de Ciências Biológicas, Universidade Federal do Pará, Pará, Brazil

    Ana Paula A. Guimarães, Adriana C. Guimarães, Diego Á. Alcântara, Luiz Raimundo Cunha, Patrícia L. Lima, Raquel C. Montenegro, Bruno M. Soares, Marucia M. Amorim & Rommel R. Burbano

  3. Faculdade de Ciências Farmacêuticas, Universidade Federal do Amazonas, Manaus, Brazil

    Marne C. Vasconcellos

  4. Universidade da Amazônia, Belém, Brazil

    Marucia M. Amorim

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  1. Ana Paula A. Guimarães
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  2. Adriana C. Guimarães
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  3. Diego Á. Alcântara
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  4. Luiz Raimundo Cunha
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  5. Patrícia L. Lima
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  6. Marne C. Vasconcellos
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  8. Bruno M. Soares
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  9. Marucia M. Amorim
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  10. Rommel R. Burbano
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Correspondence to Rommel R. Burbano .

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  1. Universidad de Oviedo, Oviedo, Spain

    L. María Sierra

  2. Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal

    Isabel Gaivão

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Guimarães, A.P.A. et al. (2014). Chromosomal Aberration Test Utilities In Vitro and In Vivo. In: Sierra, L., Gaivão, I. (eds) Genotoxicity and DNA Repair. Methods in Pharmacology and Toxicology. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-1068-7_7

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  • DOI: https://doi.org/10.1007/978-1-4939-1068-7_7

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