Use of Tandem Affinity Chromatography for Purification of Cannabinoid Receptor CB2
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Tandem affinity purification has been increasingly applied to isolation of recombinant proteins. It relies on two consecutive chromatographic steps that take advantage of the affinity tags placed at opposing ends of the target protein. This allows for efficient removal of contaminating proteins, including products of proteolytic degradation of the fusion that lack either N- or C-terminal tags. Here, we describe the use of two small affinity tags, a poly-histidine tag and a Strep-tag for expression and purification of the human cannabinoid receptor CB2, an integral membrane G protein-coupled receptor.
Key wordsHis-tag Strep-tag Dual affinity tags Membrane protein CB2 receptor StrepTactin Ni-NTA
This work was supported by the Intramural Research Program of the National Institute on Alcoholism and Alcohol Abuse, National Institutes of Health.
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