Expression, Purification, and Immobilization of Recombinant Tamavidin 2 Fusion Proteins
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Tamavidin 2 is a fungal avidin-like protein that binds biotin with high affinity. Unlike avidin or streptavidin, tamavidin 2 in soluble form is produced at high levels in Escherichia coli. In this chapter, we describe a method for immobilization and purification of recombinant proteins with the use of tamavidin 2 as an affinity tag. The protein fused to tamavidin 2 is tightly immobilized and simultaneously purified on biotinylated magnetic microbeads without loss of activity.
Key wordsAffinity tag Biotin Escherichia coli Immobilization Purification Tamavidin
The authors thank Dr. Toshihiko Komari for critical reading of the manuscript.
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