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Expression, Purification, and Immobilization of Recombinant Tamavidin 2 Fusion Proteins

  • Yoshimitsu TakakuraEmail author
  • Naomi Oka
  • Masako Tsunashima
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1177)

Abstract

Tamavidin 2 is a fungal avidin-like protein that binds biotin with high affinity. Unlike avidin or streptavidin, tamavidin 2 in soluble form is produced at high levels in Escherichia coli. In this chapter, we describe a method for immobilization and purification of recombinant proteins with the use of tamavidin 2 as an affinity tag. The protein fused to tamavidin 2 is tightly immobilized and simultaneously purified on biotinylated magnetic microbeads without loss of activity.

Key words

Affinity tag Biotin Escherichia coli Immobilization Purification Tamavidin 

Notes

Acknowledgements

The authors thank Dr. Toshihiko Komari for critical reading of the manuscript.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Yoshimitsu Takakura
    • 1
    • 2
    Email author
  • Naomi Oka
    • 1
  • Masako Tsunashima
    • 1
  1. 1.Plant Innovation CenterJapan Tobacco, Inc.IwataJapan
  2. 2.Leaf Tobacco Research CenterJapan Tobacco, Inc.OyamaJapan

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