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Simplified Protein Purification Using an Autoprocessing, Inducible Enzyme Tag

  • Aimee ShenEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1177)

Abstract

The development of affinity tags has greatly simplified protein purification procedures. A variety of affinity tags are now available to improve expression, solubility, and/or tag removal. In this chapter, we describe a method for purifying recombinant proteins expressed in Escherichia coli that uses a highly specific, inducible, C-terminal autoprocessing protease tag. This method streamlines affinity purification, cleavage, and tag separation into a one-step purification procedure, avoiding the need to remove fusion tags from target proteins with exogenous proteases. In addition to accelerating protein purification, we show that this method can enhance the expression, stability, and solubility of select proteins.

Key words

Protein affinity tag Autoprocessing Inducible Protein purification Tag cleavage Protein stability Protein solubility Protein expression 

Notes

Acknowledgement

This work was supported by NIH grant R00 GM092934/2-4 and a Vermont Immunology and Infectious Disease Center COBRE grant (P20 TT021905) to A.S.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of Microbiology and Molecular GeneticsUniversity of VermontBurlingtonUSA

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