Purification of a Recombinant Protein with Cellulose-Binding Module 3 as the Affinity Tag
Easy-to-perform and low-cost protein purification methods are in high demand for the mass production of commonly used enzymes that play an important role in bioeconomy. A low-cost and rapid recombinant protein purification system was developed using CBM3 (family 3 cellulose-binding module) as affinity tag. This protocol describes the purification of CBM3-fusion protein and tag-free protein expressed in Pichia pastoris using CBM3 as an affinity tag.
Key wordsCellulose-binding module Affinity tag Protein purification Intein Cellulose
This work was supported by a grant-in-aid from the Scientific Research Foundation for Returned Scholars, Ministry of Education of China, the Fundamental Research Funds for the Central Universities (No. KA2070000008), and the National Basic Research Program of China (No. 2011CBA00801).
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