Eosinophil Purification from Human Bone Marrow
Eosinophils are innate immune cells that are best known for their involvement in host defense against parasitic infections and in asthma and allergic diseases. In vitro characterization of the function of human eosinophils has traditionally relied on the purification of these cells from the peripheral blood as reviewed in Chapter 2. Here, we describe a newly developed protocol for the purification of eosinophils from human bone marrow.
Key wordsBone marrow eosinophils Purification Human bone marrow Cell isolation
This work was supported by the NIH Predoctoral Immunology Training Grant (T32 AI07425). TWW thanks the Mayo Medical School, Mayo Graduate School, and the Mayo Clinic Medical Scientist Training Program for providing an outstanding training environment.
- 6.Fauci A, Braunwald E, Kasper D et al (2009) Harrison’s manual of medicine, 17th edn. McGraw-Hill Companies, New York, NYGoogle Scholar