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Eosinophils pp 215-229 | Cite as

Interactions of Eosinophils with Nerves

  • Quinn R. Roth-Carter
  • David B. Jacoby
  • Zhenying Nie
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1178)

Abstract

Coculture of eosinophils and nerves is a powerful tool in determining the interactions between the two cell types. We have developed methods for culture of parasympathetic ganglia and dorsal root ganglia from humans, and we have further refined the technique to coculture with eosinophils. Here we describe methods for coculturing primary parasympathetic ganglia or dorsal root ganglia with eosinophils.

Key words

Eosinophil Parasympathetic ganglia Dorsal root ganglia Coculture Nerves Primary cell culture 

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Copyright information

© Springer Science+Business Media, New York 2014

Authors and Affiliations

  • Quinn R. Roth-Carter
    • 1
  • David B. Jacoby
    • 1
  • Zhenying Nie
    • 1
  1. 1.Pulmonary and Critical Care MedicineOregon Health and Science UniversityPortlandUSA

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