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Small RNA Library Cloning Procedure for Deep Sequencing of Specific Endogenous siRNA Classes in Caenorhabditis elegans

  • Maria C. Ow
  • Nelson C. Lau
  • Sarah E. HallEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1173)

Abstract

In recent years, distinct classes of small RNAs ranging in size from ~21 to 26 nucleotides have been discovered and shown to play important roles in a wide array of cellular functions. Because of the abundance of these small RNAs, library preparation from an RNA sample followed by deep sequencing provides the identity and quantity of a particular class of small RNAs. In this chapter we describe a detailed protocol for preparing small RNA libraries for deep sequencing on the Illumina platform from the nematode C. elegans.

Keywords

Endogenous siRNAs C. elegans RNAi 26G-siRNA 22G-siRNA Small RNA library cloning 

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of BiologySyracuse UniversitySyracuseUSA
  2. 2.Department of Biology, Rosenstiel Center of Basic Biomedical SciencesBrandeis UniversityWalthamUSA

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