Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster
- 1.2k Downloads
Endogenous small interfering RNAs (endo-siRNAs) are a newly emerged class of small regulatory RNAs. In Drosophila melanogaster, the production of endo-siRNAs depends on the RNase III enzyme Dicer-2 (Dcr-2). Loss of dcr-2 function reduces the resistance of adult flies to various stresses and shortens their life span. The mutants also exhibit alterations in carbohydrate and lipid metabolism. These findings suggest that the endo-siRNA pathway plays a protective role during exposure to stress and aging in D. melanogaster, possibly by regulating metabolic homeostasis. Here, we describe the methods that were used to discover the phenotypes associated with endo-siRNA deficiency by using dcr-2 null mutants.
KeywordsDrosophila melanogaster Endogenous siRNAs Dicer-2 Stress resistance Life span Metabolism
This work was supported by a grant from the National Research Foundation of Korea (NRF) funded by the Korea government (Ministry of Education, Science, and Technology [MEST]; 2011-0016863 to Y.S.L.) and the NRF grant funded by MEST (2012-00011, Gyeonggi-do and KISTI to S.J.H.).