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Methods for Studying the Biological Consequences of Endo-siRNA Deficiency in Drosophila melanogaster

  • Do-Hwan Lim
  • Chun-Taek Oh
  • Sung-Jun HanEmail author
  • Young Sik LeeEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1173)

Abstract

Endogenous small interfering RNAs (endo-siRNAs) are a newly emerged class of small regulatory RNAs. In Drosophila melanogaster, the production of endo-siRNAs depends on the RNase III enzyme Dicer-2 (Dcr-2). Loss of dcr-2 function reduces the resistance of adult flies to various stresses and shortens their life span. The mutants also exhibit alterations in carbohydrate and lipid metabolism. These findings suggest that the endo-siRNA pathway plays a protective role during exposure to stress and aging in D. melanogaster, possibly by regulating metabolic homeostasis. Here, we describe the methods that were used to discover the phenotypes associated with endo-siRNA deficiency by using dcr-2 null mutants.

Keywords

Drosophila melanogaster Endogenous siRNAs Dicer-2 Stress resistance Life span Metabolism 

Notes

Acknowledgements

This work was supported by a grant from the National Research Foundation of Korea (NRF) funded by the Korea government (Ministry of Education, Science, and Technology [MEST]; 2011-0016863 to Y.S.L.) and the NRF grant funded by MEST (2012-00011, Gyeonggi-do and KISTI to S.J.H.).

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.College of Life Sciences and BiotechnologyKorea UniversitySeoulSouth Korea
  2. 2.Drug Biology GroupInstitut Pasteur KoreaSeongnam-SiSouth Korea

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