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Generation of Endo-siRNAs in Xenopus laevis Oocytes

  • Sammer Alnumeir
  • Andreas WernerEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1173)

Abstract

Endogenous siRNAs (endo-siRNAs) are well documented and characterized in C. elegans and Drosophila. Endo-siRNAs can also be found in vertebrates; however, their biology is much less clear. They are thought to be produced by Dicer and to contribute to transposon silencing. Because of their generally low abundance and their similarity with miRNAs and products of physiological RNA turn-over, endo-siRNAs are difficult to investigate. Here, we report a system, oocytes from Xenopus laevis, that allows for the generation and analysis of endo-siRNAs from double-stranded RNA precursors.

Keywords

Endo-siRNA Xenopus laevis oocytes Nuclear injections 

Notes

Acknowledgment

This work has been supported by the Dunhill Medical Trust and Newcastle University.

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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.RNA Biology Group, Institute for Cell and Molecular BiosciencesNewcastle UniversityNewcastle upon TyneUK

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