Abstract
Cytokine stimulations of leukocytes many times result in transient activation of the p21 Rho family of small GTPases. The role of these molecules during cell migration and chemotaxis is well established. The traditional approach to study the activation dynamics of these proteins involves affinity pull-downs that are often cumbersome and prone to errors. Here, we describe a reagent and a method of simple “mix-and-measure” approach useful for determining the activation status of endogenous Cdc42 GTPase from cell lysates.
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References
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Acknowledgement
This work was supported by grants GM093121 (D.S., L.H.), T32GM007491 (V.M.), and GM071828 (D.C.).
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Miskolci, V., Spiering, D., Cox, D., Hodgson, L. (2014). A Mix-and-Measure Assay for Determining the Activation Status of Endogenous Cdc42 in Cytokine-stimulated Macrophage Cell Lysates. In: Vancurova, I. (eds) Cytokine Bioassays. Methods in Molecular Biology, vol 1172. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0928-5_15
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DOI: https://doi.org/10.1007/978-1-4939-0928-5_15
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-0927-8
Online ISBN: 978-1-4939-0928-5
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