Skip to main content
SpringerLink
Log in

Telomere Regulation During the Cell Cycle in Fission Yeast

  • Protocol
  • First Online:
Cell Cycle Control

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1170))

Abstract

The fission yeast Schizosaccharomyces pombe has emerged as a useful model organism to study telomere maintenance mechanisms. In this chapter, we provide detailed protocols for quantitative ChIP and BrdU incorporation analyses to investigate how fission yeast telomeres are regulated during the cell cycle by utilizing cdc25-22 synchronized cell cultures.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 159.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Lydall D (2009) Taming the tiger by the tail: modulation of DNA damage responses by telomeres. EMBO J 28(15):2174–2187

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  2. Verdun RE, Karlseder J (2007) Replication and protection of telomeres. Nature 447(7147):924–931

    Article  CAS  PubMed  Google Scholar 

  3. Price CM, Boltz KA, Chaiken MF, Stewart JA, Beilstein MA, Shippen DE (2010) Evolution of CST function in telomere maintenance. Cell Cycle 9(16):3157–3165

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  4. Moser BA, Nakamura TM (2009) Protection and replication of telomeres in fission yeast. Biochem Cell Biol 87(5):747–758

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  5. Moser BA, Subramanian L, Chang YT, Noguchi C, Noguchi E, Nakamura TM (2009) Differential arrival of leading and lagging strand DNA polymerases at fission yeast telomeres. EMBO J 28(7):810–820

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  6. Forsburg SL (2003) Growth and manipulation of S. pombe. Curr Protoc Mol Biol Chapter 13:Unit 13.16

    Google Scholar 

  7. Alfa C, Fantes P, Hyams J, McLoed M, Warbrick E (1993) Experiments with Fission Yeast. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY

    Google Scholar 

  8. Russell P, Nurse P (1986) cdc25 + functions as an inducer in the mitotic control of fission yeast. Cell 45(1):145–153

    Article  CAS  PubMed  Google Scholar 

  9. Kanoh J, Sadaie M, Urano T, Ishikawa F (2005) Telomere binding protein Taz1 establishes Swi6 heterochromatin independently of RNAi at telomeres. Curr Biol 15(20):1808–1819

    Article  CAS  PubMed  Google Scholar 

  10. Hayashi M, Katou Y, Itoh T, Tazumi A, Yamada Y, Takahashi T, Nakagawa T, Shirahige K, Masukata H (2007) Genome-wide localization of pre-RC sites and identification of replication origins in fission yeast. EMBO J 26(5):1327–1339

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  11. Nelson JD, Denisenko O, Bomsztyk K (2006) Protocol for the fast chromatin immunoprecipitation (ChIP) method. Nat Protoc 1(1):179–185

    Article  CAS  PubMed  Google Scholar 

  12. Sivakumar S, Porter-Goff M, Patel PK, Benoit K, Rhind N (2004) In vivo labeling of fission yeast DNA with thymidine and thymidine analogs. Methods 33(3):213–219

    Article  CAS  PubMed  Google Scholar 

  13. Hodson JA, Bailis JM, Forsburg SL (2003) Efficient labeling of fission yeast Schizosaccharomyces pombe with thymidine and BUdR. Nucleic Acids Res 31(21):e134

    Article  PubMed Central  PubMed  Google Scholar 

  14. Sugawara NF (1988) DNA sequences at the telomeres of the fission yeast S. pombe. Ph.D. thesis. Harvard University, Cambridge, Massachusetts

    Google Scholar 

Download references

Acknowledgement

This work was supported by NIH grant GM078253 (to T.M.N.).

Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Genetics, College of Medicine, University of Illinois at Chicago, 900 S. Ashland (M/C 669), Chicago, IL, 60607, USA

    Bettina A. Moser, Ya-Ting Chang & Toru M. Nakamura

Authors
  1. Bettina A. Moser
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ya-Ting Chang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Toru M. Nakamura
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Toru M. Nakamura .

Editor information

Editors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Drexel University College of Medicine, Philadelphia, Pennsylvania, USA

    Eishi Noguchi

  2. Dept of Biochemistry & Molecular Biology, Drexel University College of Medicine, Philadelphia, USA

    Mariana C. Gadaleta

Rights and permissions

Reprints and permissions

Copyright information

© 2014 Springer Science+Business Media New York

About this protocol

Cite this protocol

Moser, B.A., Chang, YT., Nakamura, T.M. (2014). Telomere Regulation During the Cell Cycle in Fission Yeast. In: Noguchi, E., Gadaleta, M. (eds) Cell Cycle Control. Methods in Molecular Biology, vol 1170. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0888-2_22

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-0888-2_22

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0887-5

  • Online ISBN: 978-1-4939-0888-2

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation