Abstract
Numerous cell cycle-regulating proteins are controlled by protein degradation. Recent work shows that ubiquitination-dependent proteolysis plays an important role in once-per-cell cycle control of DNA replication. Cdt1 is a licensing factor essential for assembling the pre-replicative complex on replication origins. Cdt1 is present in G1 phase, but after S phase ubiquitin-mediated proteolysis maintains Cdt1 at low levels. This is important to prevent the re-replication of chromosomal DNA. The cell cycle-dependent degradation of Cdt1 can be monitored by dual staining of the cell nuclei with antibodies against Cdt1- and S/G2-phase marker proteins, such as cyclin A or geminin.
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This work was financially supported by JSPS KAKENHI and MEXT KAKENHI, Grant in Aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan.
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Shiomi, Y., Suenaga, N., Tanaka, M., Hayashi, A., Nishitani, H. (2014). Imaging Analysis of Cell Cycle-Dependent Degradation of Cdt1 in Mammalian Cells. In: Noguchi, E., Gadaleta, M. (eds) Cell Cycle Control. Methods in Molecular Biology, vol 1170. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0888-2_18
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DOI: https://doi.org/10.1007/978-1-4939-0888-2_18
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