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Measuring Freezing Tolerance: Survival and Regrowth Assays

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Plant Cold Acclimation

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1166))

Abstract

Screening plants for freezing tolerance under tightly controlled conditions is an invaluable technique for studying freezing tolerance and selecting for improved winterhardiness. Artificial freezing tests of cereal plants historically have used isolated crown and stem tissue prepared by “removing all plant parts 3 cm above and 0.5 cm below the crown tissue” (Fowler et al., Crop Sci 21:896–901, 1981). Here, we describe a method of conducting freezing tolerance tests using intact plants grown in small horticultural containers, including suggested methods for collecting and analyzing the data.

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Author information

Authors and Affiliations

  1. USDA-ARS, Wheat Genetics Unit, Washington State University, 209 Johnson Hall, Pullman, WA, 99164, USA

    Daniel Z. Skinner & Kimberly Garland-Campbell

Authors
  1. Daniel Z. Skinner
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  2. Kimberly Garland-Campbell
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Corresponding author

Correspondence to Daniel Z. Skinner .

Editor information

Editors and Affiliations

  1. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    Dirk K. Hincha

  2. Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam, Germany

    Ellen Zuther

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Skinner, D.Z., Garland-Campbell, K. (2014). Measuring Freezing Tolerance: Survival and Regrowth Assays. In: Hincha, D., Zuther, E. (eds) Plant Cold Acclimation. Methods in Molecular Biology, vol 1166. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0844-8_2

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  • DOI: https://doi.org/10.1007/978-1-4939-0844-8_2

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0843-1

  • Online ISBN: 978-1-4939-0844-8

  • eBook Packages: Springer Protocols

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