Pseudogenes pp 63-73 | Cite as

Methods of Identification of Pseudogenes Based on Functionality: Hybridization of 18S rRNA and mRNA During Translation

  • Chuanhua Xing
Part of the Methods in Molecular Biology book series (MIMB, volume 1167)


Protein-coding sequences are characterized by a period-3 free energy signal that arises from the interaction between the 3′-terminal nucleotides of the 18S rRNA and the mRNA. Such a signal is not present in noncoding sequences such as introns and intergenic regions and can be used for pseudogene identification.

Key words

Hybridization of 18S rRNA and mRNA Secondary structure Free energy Cumulative sinusoidal wave Period-3 signal Amplitude Phase Pseudogene prediction 


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  1. 1.Department of BiostatisticsBoston University, School of Public HealthBostonUSA

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