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piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe

Part of the Methods in Molecular Biology book series (MIMB,volume 1163)

Abstract

Transposon-mediated insertional mutagenesis is a powerful tool for genetic screens. We have developed a piggyBac transposon-based mutagenesis system for the fission yeast Schizosaccharomyces pombe. Here, we describe in detail the procedure for inducing and selecting transpositions, and two protocols for identifying the transposon insertion sites, one using inverse PCR to identify the insertion sites in individual mutants, and the other using high-throughput sequencing to reveal the insertion sites in a mutant pool containing hundreds to thousands of mutants.

Key words

  • piggyBac
  • Transposon
  • Mutagenesis
  • Fission yeast
  • Schizosaccharomyces pombe

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  • DOI: 10.1007/978-1-4939-0799-1_16
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Correspondence to Li-Lin Du .

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Li, J., Du, LL. (2014). piggyBac Transposon-Based Insertional Mutagenesis for the Fission Yeast Schizosaccharomyces pombe . In: Xiao, W. (eds) Yeast Protocols. Methods in Molecular Biology, vol 1163. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-0799-1_16

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  • DOI: https://doi.org/10.1007/978-1-4939-0799-1_16

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-0798-4

  • Online ISBN: 978-1-4939-0799-1

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